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BJA Advance Access published online on March 23, 2009
British Journal of Anaesthesia, doi:10.1093/bja/aep023
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© The Author [2009]. Published by Oxford University Press on behalf of The Board of Directors of the British Journal of Anaesthesia. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournal.org

Effects of bupivacaine and ropivacaine on field potential in rat hippocampal slices

J.-W. Yi1,*, B.-J. Lee1, D.-O. Kim1, S.-W. Park2, Y.-K. Choi2, H.-K. Chang3, C.-J. Kim3 and J.-H. Park4

1 Department of Anaesthesiology, East-West Neo Medical Center, Seoul, Republic of Korea
2 Department of Anaesthesiology
3 Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Republic of Korea
4 Division of Transplantation and Vascular Surgery, Department of Surgery, Ilsanpaik Hospital, Inje University, College of Medicine, Gyeonggi-do, Republic of Korea

* Corresponding author. E-mail: mdyjw{at}hanmail.net

Background: In spite of more than 20 yr of research, the mechanism whereby local anaesthetics act on the brain to mediate anaesthesia still remains unclear. Furthermore, the effect of local anaesthetics on neuronal excitability and synaptic transmission in the hippocampus has not been reported. Thus, the purpose of the present study was to find out the differences between the local anaesthetics, bupivacaine and ropivacaine, in their actions on synaptic transmission of brain in the context of hippocampal field potential.

Methods: Brains were removed from 3- to 4-week-old rats and transverse slices (300 µm thick) were prepared using a microslicer. A slice was then placed on the centre on a multielectrode dish probe. To record evoked field potentials at 64 sites, a pair of single planar microelectrodes delivering bipolar constant current pulses (45–90 µA, 0.1 ms) was applied. Electrophysiological recordings were measured using the 64-channel multielectrode dish.

Results: The amplitude of field potential in the rat CA1 region was inhibited by both bupivacaine and ropivacaine. The inhibitory effects of bupivacaine and ropivacaine on field potential amplitudes in CA1 were similar. For bupivacaine 10 µg ml–1, inhibited field potentials were incompletely recovered; in contrast, for 10 ropivacaine µg ml–1, inhibited field potentials were completely recovered after washing out with incubation solution.

Conclusions: Inhibitory effects of bupivacaine and ropivacaine on hippocampal field potential amplitude and recovery rate after washout after bupivacaine or ropivacaine treatment represent the underlying mechanisms of the systemic toxicity of local anaesthetics.

Keywords: anaesthetics local, bupivacaine; anaesthetics local, ropivacaine; brain, hippocampus; ions, ion channels, voltage-gated


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