Skip Navigation



BJA Advance Access published online on February 4, 2005
British Journal of Anaesthesia, doi:10.1093/bja/aei080
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
94/4/479    most recent
aei080v1
Right arrow E-Letters: Submit a response to the article
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Dinse, A.
Right arrow Articles by Weigt, H. U.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dinse, A.
Right arrow Articles by Weigt, H. U.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Board of Management and Trustees of the British Journal of Anaesthesia 2005. All rights reserved. For Permissions, please e-mail: journal.permissions@oupjournals.org
Accepted December 13, 2004

Laboratory Investigation

Xenon reduces glutamate-, AMPA-, and kainate-induced membrane currents in cortical neurones

A. Dinse 1, K. J. Föhr 1*, M. Georgieff 1, C. Beyer 2, A. Bulling 3, and H. U. Weigt 1

1 Clinic for Anesthesiology, Ulm, Germany
2 Department of Anatomy, Tübingen, Germany
3 flyion GmbH, Tübingen, Germany

* To whom correspondence should be addressed.
K. J. Föhr, E-mail: karl.foehr{at}medizin.uni-ulm.de


  Abstract

Background. The anaesthetic, analgesic, and neuroprotective effects of xenon (Xe) are believed to be mediated by a block of the NMDA (N-methyl-D-aspartate) receptor channel. Interestingly, the clinical profile of the noble gas differs markedly from that of specific NMDA receptor antagonists. The aim of this study was, therefore, to investigate whether Xe might be less specific, also inhibiting the two other subtypes of glutamate receptor channels, such as the {alpha}-amino-3-hydroxy-5-methyl-4-isoxazolole propionate (AMPA) and kainate receptors.

Methods. The study was performed on voltage-clamped cortical neurones from embryonic mice and SH-SY5Y cells expressing GluR6 kainate receptors. Drugs were applied by a multi-barreled fast perfusion system.

Results. Xe, dissolved at approximately 3.45 mM in aqueous solution, diminished the peak and even more the plateau of AMPA and glutamate induced currents. At the control EC50 value for AMPA (29 µM) these reductions were by about 40 and 56% and at 3 mM glutamate the reductions were by 45 and 66%, respectively. Currents activated at the control EC50 value for kainate (57 µM) were inhibited by 42%. Likewise, Xe showed an inhibitory effect on kainate-induced membrane currents of SH-SY5Y cells transfected with the GluR6 subunit of the kainate receptor. Xe reduced kainate-induced currents by between 35 and 60%, depending on the kainate concentration.

Conclusions. Xe blocks not only NMDA receptors, but also AMPA and kainate receptors in cortical neurones as well as GluR6-type receptors expressed in SH-SY5Y cells. Thus, Xe seems to be rather non-specific as a channel blocker and this may contribute to the analgesic and anaesthetic potency of Xe.

Keywords: anaesthetics volatile, xenon; measurement techniques, electrophysiology; model, brain cortical neurones; model, neuroblastoma cells; pharmacology, glutamate ligand gated ion channel; receptors, amino acid, AMPA; receptors, amino acid, kainate; receptors; amino acid, NMDA.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Anesth. Analg.Home page
E. Chakkarapani, M. Thoresen, C. E. Hobbs, K. Aquilina, X. Liu, and J. Dingley
A Closed-Circuit Neonatal Xenon Delivery System: A Technical and Practical Neuroprotection Feasibility Study in Newborn Pigs
Anesth. Analg., August 1, 2009; 109(2): 451 - 460.
[Abstract] [Full Text] [PDF]

Home page
 Br J AnaesthHome page
A. LacKamp, G.-C. Zhang, L.-M. Mao, E. E. Fibuch, and J. Q. Wang
Loss of surface N-methyl-D-aspartate receptor proteins in mouse cortical neurones during anaesthesia induced by chloral hydrate in vivo
Br. J. Anaesth., April 1, 2009; 102(4): 515 - 522.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
D. P. D'Agostino, D. G. Colomb Jr., and J. B. Dean
Effects of hyperbaric gases on membrane nanostructure and function in neurons
J Appl Physiol, March 1, 2009; 106(3): 996 - 1003.
[Abstract] [Full Text] [PDF]

Home page
 Br J AnaesthHome page
M. Haines, L. M. Mao, L. Yang, A. Arora, E. E. Fibuch, and J. Q. Wang
Modulation of AMPA receptor GluR1 subunit phosphorylation in neurons by the intravenous anaesthetic propofol
Br. J. Anaesth., May 1, 2008; 100(5): 676 - 682.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
H. U. Weigt, O. Adolph, M. Georgieff, E. M. Georgieff, and K. J. Fohr
Evidence That Xenon Does Not Produce Open Channel Blockade of the NMDA Receptor
J Neurophysiol, April 1, 2008; 99(4): 1983 - 1987.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
H. N. David, B. Haelewyn, C. Rouillon, M. Lecoq, L. Chazalviel, G. Apiou, J.-J. Risso, M. Lemaire, and J. H. Abraini
Neuroprotective effects of xenon: a therapeutic window of opportunity in rats subjected to transient cerebral ischemia
FASEB J, April 1, 2008; 22(4): 1275 - 1286.
[Abstract] [Full Text] [PDF]

Home page
 StrokeHome page
C. Hobbs, M. Thoresen, A. Tucker, K. Aquilina, E. Chakkarapani, and J. Dingley
Xenon and Hypothermia Combine Additively, Offering Long-Term Functional and Histopathologic Neuroprotection After Neonatal Hypoxia/Ischemia
Stroke, April 1, 2008; 39(4): 1307 - 1313.
[Abstract] [Full Text] [PDF]

Home page
 Anesth. Analg.Home page
J. Dingley, C. Hobbs, J. Ferguson, J. Stone, and M. Thoresen
Xenon/Hypothermia Neuroprotection Regimes in Spontaneously Breathing Neonatal Rats After Hypoxic-Ischemic Insult: The Respiratory and Sedative Effects
Anesth. Analg., March 1, 2008; 106(3): 916 - 923.
[Abstract] [Full Text] [PDF]

Home page
 Anesth. Analg.Home page
J. Benrath, C. Kempf, M. Georgieff, and J. Sandkuhler
Xenon Blocks the Induction of Synaptic Long-Term Potentiation in Pain Pathways in the Rat Spinal Cord In Vivo
Anesth. Analg., January 1, 2007; 104(1): 106 - 111.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.