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BJA Advance Access published online on February 11, 2005
British Journal of Anaesthesia, doi:10.1093/bja/aei077
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© The Board of Management and Trustees of the British Journal of Anaesthesia 2005. All rights reserved. For Permissions, please e-mail: journal.permissions@oupjournals.org
Accepted November 10, 2004

Laboratory Investigation

Anoxic depolarization of rat hippocampal slices is prevented by thiopental but not by propofol or isoflurane

R. Sasaki 1, K. Hirota 1*, S. H. Roth 2, and M. Yamazaki 1

1 Department of Anaesthesiology, Toyama Medical and Pharmaceutical University of Medicine, 2630 Sugitani, Toyama, 930-0194, Japan
2 Department of Pharmacology, Faculty of Medicine, University of Calgary, 3330 Hospital Drive NW, Calgary, Alberta T2N 4N1, Canada; Department of Therapeutics, Faculty of Medicine, University of Calgary, 3330 Hospital Drive NW, Calgary, Alberta T2N 4N1, Canada; Department of Anaesthesia, Faculty of Medicine, University of Calgary, 3330 Hospital Drive NW, Calgary, Alberta T2N 4N1, Canada

* To whom correspondence should be addressed.
K. Hirota, E-mail: koki{at}ms.toyama-mpu.ac.jp


  Abstract

Background. There is strong evidence to suggest that anoxic depolarization (AD) is an important factor in hypoxia/ischaemia-induced neural damage. Treatments that prevent the occurrence of AD may be useful in providing neuronal protection against hypoxia. The current study was designed to determine whether general anaesthetics which have been suggested to ‘induce prophylaxis’ against hypoxia can attenuate the incidence of AD.

Methods. The effects of anoxia (3 min) on evoked extracellularly recorded field potentials of CA1 neurons in rat hippocampal slices were assessed in the absence and presence of the i.v. general anaesthetics thiopental and propofol and the volatile anaesthetic isoflurane.

Results. In the absence of anaesthetics, AD occurred in 81% of the preparations tested. Thiopental (2x10-4 M) significantly reduced the incidence of AD (16%, P=0.0006). In comparison, propofol (2x10-4 M) and isoflurane (1.5 vol%) were ineffective (69% and 60%, respectively). Furthermore, in the presence of thiopental, the population spike amplitude recovered with and without AD (90% and 94% of pre-anoxic value, respectively) following 3 min anoxia.

Conclusion. The prophylactic effect of thiopental against hypoxia might be induced, in part, by preventing the generation of AD.

Keywords: anaesthetics, i.v., thiopental; anaesthetics, i.v., propofol; anaesthetics, volatile, isoflurane; measurement techniques, electrophysiology; model brain slice, hippocampus.
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