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British Journal of Anaesthesia, 2003, Vol. 90, No. 2 233-237
© 2003 The Board of Management and Trustees of the British Journal of Anaesthesia

Short Communications

Sevoflurane anaesthesia does not induce the formation of sister chromatid exchanges in peripheral blood lymphocytes of children

T. Krause*,1, J. Scholz1, L. Jansen1, H. Boettcher1, C. Koch1, F. Wappler1 and J. Schulte am Esch2

1 Christian-Albrechts-University Kiel, Brunswiker Strasse 10, D-24105 Kiel, Germany. 2 University-Hospital Hamburg Eppendorf, Martinistrasse 52, D-20246 Hamburg, Germany

Corresponding author: Klinik und Poliklinik für Anästhesiologie, Universitätsklinikum Hamburg-Eppendorf, Martinistrasse 52, D-20246 Hamburg, Germany. E-mail: tkrause@uke.uni-hamburg.de

Background. Compound A, a degradation product of sevoflurane, has been demonstrated to induce sister chromatid exchanges (SCE) in Chinese hamster ovary cells in vitro as a marker for possible genotoxicity. We investigated the formation of SCE in mitogen-stimulated T-lymphocytes of 40 children undergoing sevoflurane anaesthesia for minor surgical procedures.

Methods. Anaesthesia was induced by inhalation of up to 8% sevoflurane and maintained at 2.5–3% in oxygen/nitrous oxide (65/35%) at a fresh gas flow of 3 litre min–1. Soda lime (humidity 12–15%) was used as a carbon dioxide absorbent. Blood was drawn directly before induction and after termination of anaesthesia. Twenty-five second division metaphases of mitogen-stimulated T-lymphocytes per blood sample were screened for SCE rates using standard techniques.

Results. Average duration of anaesthesia was 49.6 (SD 24.0) min. Before anaesthesia induction, 7.93 (1.23) SCE per metaphase were determined. After sevoflurane anaesthesia [1.40 (0.77) MAC h] 7.92 (1.19) SCE per metaphase were observed. Additionally, no differences were evident between male or female children.

Conclusion. Short-term administration of sevoflurane anaesthesia did not induce SCE in T-lymphocytes of children. No indication for a possible genotoxic effect has been observed.

Br J Anaesth 2003; 90: 233–5


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