British Journal of Anaesthesia, Vol 80, Issue 5 644-648, Copyright © 1998 by The Board of Management and Trustees of the British Journal of Anaesthesia
T. Kikuchi, Y. Wang, K. Sato and F. Okumura
Using in vivo microdialysis, we have investigated the effects of propofol
on acetylcholine (ACh) release from various regions of the rat brain.
Propofol 25 and 50 mg kg-1 i.p. decreased basal ACh release from the
frontal cortex by 70% and 85%, respectively. Propofol 25 and 50 mg kg-1
i.p. decreased basal ACh release from the hippocampus by 47% and 72%,
respectively. However, in rat striatum, propofol 25 mg kg-1 i.p. did not
affect basal ACh release and 50 mg kg-1 i.p. produced slight inhibition of
basal ACh release (by 19%) only in the second sample after i.p. injection.
In addition, we also examined the pharmacological mechanisms mediating the
interaction between propofol and a gamma- aminobutyric acid A (GABAA)
receptor complex. In the rat hippocampus, local application of bicuculline
1 mumol litre-1, a GABAA receptor antagonist, significantly antagonized the
inhibitory effects of propofol 50 mg kg-1 i.p. on basal ACh release. In the
rat frontal cortex, local application of bicuculline 1 mumol litre-1 did
not antagonize the inhibitory effects of propofol 50 mg kg-1 i.p. on basal
ACh release, while systemic application of bicuculline 1 mg kg-1 i.p.
significantly antagonized the inhibitory effects of propofol 50 mg kg-1
i.p. These results suggest that propofol has powerful depressant effects on
ACh release from the rat frontal cortex and hippocampus but not from the
striatum, indicating that propofol has a "region- selective" anaesthetic
action. Further, these results suggest that the inhibitory effects of
propofol in the rat hippocampus may involve "intra" hippocampal GABAA
receptors while the inhibitory effects in the rat frontal cortex may be
mediated by GABAA receptors other than "intra" frontal cortex GABAA
receptors.
LABORATORY INVESTIGATIONS
In vivo effects of propofol on acetylcholine release from the frontal cortex, hippocampus and striatum studied by intracerebral microdialysis in freely moving rats
Department of Anaesthesiology, Yokohama City University School of Medicine, Yokohama 236, Japan
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