British Journal of Anaesthesia, 2003, Vol. 91, No. 4 541-545
© 2003 The Board of Management and Trustees of the British Journal of Anaesthesia
Laboratory Investigations |
Heritable differences in respiratory drive and breathing pattern in mice during anaesthesia and emergence
Department of Environmental Health Sciences/Division of Physiology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, USA* Corresponding author: Department of Anaesthesiology and Critical Care Medicine, University of Essen, Hufelandstrasse 55, D-45122 Essen, Germany. E-mail: harald.groeben@uni-essen.de
Presented in part on May 18–22, 2002, at the ATS annual meeting, Atlanta, GA, USA.
Background. Postanaesthetic hypoxia and ischaemia can lead to postoperative morbidity and mortality. We studied the effect of isoflurane anaesthesia in two inbred mouse strains known for phenotypic differences in breathing pattern and respiratory drive during carbon dioxide challenge and their first-generation offspring (F1).
Methods. Using whole body plethysmography, we assessed respiratory rate (RR) and pressure amplitude (Amp) in male B6 (high responder to hypercapnia), C3 (low responder), and F1 mice at rest, during anaesthesia with isoflurane, and during recovery from anaesthesia. At each time point, the magnitude and pattern of breathing were determined during hypercapnic challenge (FICO2 = 0.08). Data (mean (SD)) were analysed by generalized ANOVA with post hoc Bonferroni’s correction (P<0.05).
Results. During isoflurane anaesthesia, strain differences between B6 and C3 mice in RR were obscured while differences in Amp persisted. In contrast to baseline RR responses to carbon dioxide were significantly reduced at 0.5 MAC (increase in RR: 175 (33) bpm, 147 (44) bpm, 127 (33) bpm, for B6, C3, and F1 strains respectively) and completely blocked at 1.5 MAC (change in RR: –3 (10) bpm, –2 (1) bpm, –4 (5) bpm, for B6, C3, and F1 strains, respectively). During recovery, B6 mice showed a significant increase in RR (77 (33) bpm; P<0.0001) as well as in Amp. This was not observed in either C3 (–22 (31) bpm) or F1 mice (23 (51) bpm).
Conclusion. Isoflurane anaesthesia abolished the strain differences in respiratory drive between B6, C3, and F1 mice. However, during recovery from anaesthesia, significant strain variation in respiratory drive reappeared and was more pronounced compared with pre-anaesthetic levels. These results suggested, that genetic differences may have minimal contribution to decreased respiratory drive during anaesthesia, but may be a major risk factor for post-operative hypoventilation and the associated morbidity and mortality.
Br J Anaesth 2003; 91: 541–5
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