British Journal of Anaesthesia, 2003, Vol. 91, No. 3 390-396
© 2003 The Board of Management and Trustees of the British Journal of Anaesthesia
Laboratory Investigations |
Desflurane affords greater protection than halothane against focal cerebral ischaemia in the rat
1 Laboratory of Experimental Anaesthesiology and Cellular Physiology, University of Caen, UPRES EA 3212, Département d’Anesthésie Réanimation, Centre Hospitalier Universitaire (CHU), Côte de Nacre, Caen, France. 2 Laboratory of Neuronal Death, Neuroprotection and Neurotransmission, University of Caen, CNRS, UMR-6551, CYCERON Center, Boulevard Henri Becquerel, BP 5229, F-14074 Caen Cedex, France
Corresponding author
Background. We studied the potential neuroprotective effects of halothane and desflurane, compared with the awake state, on infarct size following 2 h of intraluminal middle cerebral artery occlusion (MCAo) and 22 h of reperfusion.
Methods. Male Sprague–Dawley rats were anaesthetized with desflurane or halothane, intubated, and mechanically ventilated. Mean arterial pressure (MAP), blood gases, and pH were controlled. Body temperature was maintained at 37.5–38°C. Animals were assigned to one of four groups according to the anaesthetic type (halothane or desflurane) and the duration of anaesthesia: ‘short-duration’, during the preparation only; ‘long-duration’, during both preparation and ischaemia. Twenty-four hours after MCAo, infarcts were visualized by staining with 2,3,5-triphenyltetrazolium chloride. Two additional groups of rats were subjected to the same protocol as that of long-duration halothane and long-duration desflurane with additional pericranial temperature measurements made.
Results. Physiological parameters were comparable between the groups but MAP was higher (P<0.0001) in the short-duration groups. In the short-duration groups, cerebral infarct volumes were not significantly different between anaesthetics (short-duration halothane: 288 (61) mm3, mean (SD); short-duration desflurane: 269 (71) mm3, P>0.56). Compared with the awake state (short-duration groups), halothane and desflurane significantly reduced infarct volumes (long-duration halothane: 199 (54) mm3, P<0.0047 vs short-duration halothane; long-duration desflurane: 121 (55) mm3, P<0.0001 vs short-duration desflurane). The mean infarct volume in the long-duration desflurane group was significantly lower than that in the long-duration halothane group (P<0.0053). Pericranial temperatures were similar in the desflurane and halothane long-duration groups (P>0.17).
Conclusions. In rats, desflurane-induced neuroprotection against focal cerebral ischaemia was greater than that conferred by halothane.
Br J Anaesth 2003; 91: 390–6
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  B. Zhang, X. Wei, X. Cui, H. Zhou, W. Ding, and W. Li Desflurane Affords Greater Protection Than Halothane in the Function of Mitochondria Against Forebrain Ischemia Reperfusion Injury in Rats Anesth. Analg., April 1, 2008; 106(4): 1242 - 1249. [Abstract] [Full Text] [PDF]
|
|