British Journal of Anaesthesia, 2001, Vol. 86, No. 6 853-858
© 2001 The Board of Management and Trustees of the British Journal of Anaesthesia
Propofol inhibits FMLP-stimulated phosphorylation of p42 mitogen-activated protein kinase and chemotaxis in human neutrophils
Department of Anesthesiology and Critical Care Medicine, Kyushu University, Fukuoka, Japan*Corresponding author: 3-1-1 Maidashi, Higashi-ku, Fukuoka 812–8582, Japan
Propofol is used in the peri-operative setting and may affect some neutrophil functions. The effects of propofol on the function and intracellular signal transduction systems of neutrophils is controversial. Mitogen-activated protein kinase families (MAPKs) are members of the intracellular signal-transducing systems in eukaryotes. MAPKs have been shown to be involved in neutrophil chemotaxis by the use of PD98059, the specific inhibitor of MAPK/ERK kinase (MEK). The effects of propofol in dimethyl sulfoxide on phosphorylation of MAPKs and chemotaxis were investigated in human neutrophils. Isolated neutrophils (2x107 cells per ml) from healthy volunteers were incubated with propofol (2–500 µM) and stimulated by N-formyl-L-methionyl-phenylalanine (FMLP) (100 nM). The effects of propofol on the phosphorylation of p44/42 MAPK were investigated by immunoblotting. The effects of FMLP (1 µM) on chemotaxis were investigated with the under-agarose method. The phosphorylation of p42 MAPK and chemotaxis stimulated by FMLP were both inhibited by propofol at clinically relevant concentrations (
10 and 20 µM respectively). PD98059 (50 µM) also inhibited chemotaxis stimulated by FMLP, suggesting the involvement of p42 MAPK in the response. Propofol might therefore inhibit human neutrophil chemotaxis, at least in part, by suppressing the p44/42 MAPK pathway.
Br J Anaesth 2001; 86: 853–8
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  G. Cohen, D. Ilic, J. Raupachova, and W. H. Horl Resistin Inhibits Essential Functions of Polymorphonuclear Leukocytes J. Immunol., September 15, 2008; 181(6): 3761 - 3768. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Wang, Z. Xue, Q. Wang, X. Feng, and Z. Shen Propofol Protects Hepatic L02 Cells from Hydrogen Peroxide-Induced Apoptosis via Activation of Extracellular Signal-Regulated Kinases Pathway Anesth. Analg., August 1, 2008; 107(2): 534 - 540. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. T. Kneass and R. B. Marchase Protein O-GlcNAc Modulates Motility-associated Signaling Intermediates in Neutrophils J. Biol. Chem., April 15, 2005; 280(15): 14579 - 14585. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. C. Tsai, M. L. Rodriguez, K. S. Young, J. C. Deng, V. J. Thannickal, K. Tateda, M. B. Hershenson, and T. J. Standiford Azithromycin Blocks Neutrophil Recruitment in Pseudomonas Endobronchial Infection Am. J. Respir. Crit. Care Med., December 15, 2004; 170(12): 1331 - 1339. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. S. Hii, D. S. Anson, M. Costabile, V. Mukaro, K. Dunning, and A. Ferrante Characterization of the MEK5-ERK5 Module in Human Neutrophils and Its Relationship to ERK1/ERK2 in the Chemotactic Response J. Biol. Chem., November 26, 2004; 279(48): 49825 - 49834. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Moghaddami, M. Costabile, P. K. Grover, H. P. A. Jersmann, Z. H. Huang, C. S. T. Hii, and A. Ferrante Unique Effect of Arachidonic Acid on Human Neutrophil TNF Receptor Expression: Up-Regulation Involving Protein Kinase C, Extracellular Signal-Regulated Kinase, and Phospholipase A2 J. Immunol., September 1, 2003; 171(5): 2616 - 2624. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Gouwy, S. Struyf, F. Mahieu, W. Put, P. Proost, and J. Van Damme The Unique Property of the CC Chemokine Regakine-1 to Synergize with Other Plasma-Derived Inflammatory Mediators in Neutrophil Chemotaxis Does Not Reside in Its NH2-Terminal Structure Mol. Pharmacol., July 1, 2002; 62(1): 173 - 180. [Abstract] [Full Text] [PDF]
|
|