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British Journal of Anaesthesia, 1987, Vol. 59, No. 6 760-771
© 1987 The Board of Management and Trustees of the British Journal of Anaesthesia

other

TISSUE RESPONSES TO HYPEROXIA

Biochemistry and Pathology

N. R. WEBSTER, M.B., CH.B., PH.D., F.F.A.R.C.S.*, C. TOOTHILL, PH.D., F.R.C.S. and P. N. COWEN, M.B., CH.B., PH.D., F.R.C.PATH.{dagger}

Department of Chemical Pathology, University of Leeds Leeds 2.

Correspondence to N.W., Harrow.

*Clinical Research Centre, Division of Anaesthesia, Harrow, Middlesex HA1 3UJ.

{dagger}Department of Pathology, University of Leeds, Leeds 2.

An animal model was established to study the toxic effects of hyperoxia and the consequent changes in intracellular antioxidant status. Super-oxide dismutase, catalase and glutathione peroxidase activities were measured in erythro-cytes, liver and lung, in addition to cellular glutathione concentrations and its associated metabolism. Overt cellular damage was assessed biochemically by measurement of lipid peroxidation, hydrogen peroxide-induced haemolysis and osmotic fragility. Pathological changes were assessed by light and electron microscopy. Up to 11 days exposure of rats to 80% oxygen was not lethal, but resulted in overt cellular damage to red blood cells (haemoglobin concentration decreased from 13.8±1.4 (SD) g dl–1 to 12.4±0.5g dl–1; hydrogen peroxide-induced haemolysis increased from 7.7±1.6% to 75.1 ±13.5% after 11 days of hyperoxia) and to cells of lung (4-fold increase in lipid peroxida-tion) as well as a biochemical adaptation to the increased concentration of oxygen metabolites (superoxide dismutase increased 3-fold, catalase 5-fold and glutathione peroxidase 2-fold). It is suggested that red cell hydrogen peroxide-induced haemolysis and reduced glutathione concentration may be useful indicators of oxidant stress in the clinical situation.


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