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British Journal of Anaesthesia, 1985, Vol. 57, No. 8 796-802
© 1985 The Board of Management and Trustees of the British Journal of Anaesthesia

research-article

DETERMINATION OF THE PARTIAL PRESSURE OF HALOTHANE (OR ISOFLURANE) IN BLOOD

A. M. ZBINDEN, M.D., F. J. FREI, M.D., B. FUNK, M.D.,PH.D, D. A. THOMSON, M.D.,PH.D and D. WESTENSKOW, PH.D

Department of Anaesthesia, University of Basel/Kantonsspital CH-4031 Basel, Switzerland
Department of Anaesthesia, University of Utah, Salt Lake City, Utah, U.S.A.

A gas chromatographic method is described for the direct quantitative determination of the partial pressure of halothane {or isoflurane) in blood as well as the blood-gas partition coefficient. A head space technique and a flame ionization detector were used. Standard blood was obtained by equilibrating patients' blood with known gas concentrations in a tonometer. Using an infra-red analyser to measure the halothane gas concentration in the tonometer and within the anaesthetic system allowed for the direct comparison of the partial pressure in blood to the partial pressure in the inspired gas. Technical problems associated with this procedure, and with comparable methods, are discussed.


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